Journal: Alzheimer's Research & Therapy
Article Title: An improved immunoassay detects Aβ oligomers in human biofluids: their CSF levels rise with tau and phosphotau levels
doi: 10.1186/s13195-025-01802-x
Figure Lengend Snippet: Validation of plate-based 71A1/3D6 oAβ assay consistency over time. Correlation of 71A1/3D6 oAβ readouts from 8 individual human plasmas ( A ) and 8 individual human CSFs ( B ) across three independent assay runs, with oAβ concentrations from experiment 1 on the x-axis and oAβ concentrations from experiments 2 and 3 on the y-axis. C Correlation of oAβ measurements of 11 individual human CSFs on the previous bead-based assay versus on the new plate-based assay, demonstrating consistency between the assay platforms. D plate-based 71A1/3D6 oAβ measurement of post IP’ed supernatants from one human (AD) brain soaking extract immunodepleted with 71A1 conjugated to either C1 streptavidin Dynabeads or protein G Dynabeads or Streptavidin microplates
Article Snippet: Wells of Streptavidin Coated High-Capacity Microplates (Pierce) were coated with 50 μL of a 6 μg/mL solution of biotinylated 71A1 in Oligomer Aβ Assay Buffer (see Methods) (proprietary, made by EMD Millipore 02–0607-00 specifically for the 71A1/3D6 assay).
Techniques: Biomarker Discovery, Bead-based Assay